Macromolecular photoaffinity labeling wth radioactive photoactivable heterobifunctional reagents

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(82)90481-X· OSTI ID:7089914

Ji, T H; Ji, I

Three new photoactivable heterobifunctional reagents which can be radioiodinated have been synthesized. One of the radioactive reagents, the N-hydroxysuccinimide ester of 4-azidosalicylic acid was coupled to concanavalin A and the lectin derivative was used for the photoaffinity labeling of the lectin receptor on the erythrocyte ghost membrane.

• Research Organization: Univ. of Wyoming, Laramie
• OSTI ID: 7089914
• Journal Information: Anal. Biochem.; (United States), Vol. 121:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
RADIORECEPTOR ASSAY
RECEPTORS
LABELLING
AZIDO COMPOUNDS
CHEMICAL BONDS
COMPLEXES
CONCANAVALIN
ERYTHROCYTES
IODINE 125
LECTINS
LIGANDS
PHOTOCHEMICAL REACTIONS
RADIOCHEMICAL ANALYSIS
SALICYLIC ACID
AGGLUTININS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HEMAGGLUTININS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques