Brain transfer RNA. II. Analysis of modified nucleosides

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00965070· OSTI ID:7089896

Chaudhary, K D; Carrier-Malhotra, L; Murthy, M R

Transfer RNAs were isolated from rat and calf brains and their nucleosides were analysed by tritium derivative technique. Qualitative changes in the minor nucleoside components were compared on the fluorograms which showed differences in the intensities of spots. Cerebellar and cortical tRNAs were also compared, but revealed no significant quantitative differences in their methylated constituants despite 60% higher methyltransferase activity observed in cerebellum compared to cerebral cortex. An overall similarity was noticed between the relative proportions of the major and minor nucleosides of tRNAs derived from rat or calf brain, expressed as mol%. Brain tRNA was also analysed by two-dimensional polyacrylamide gel electrophoresis which showed qualitative and quantitative changes during postnatal development.

• Research Organization: Departement de Biochimie Faculte de Medecine Universite Laval Quebec, Canada
• OSTI ID: 7089896
• Journal Information: Neurochem. Res.; (United States), Vol. 7:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
TRANSFER RNA
STRUCTURAL CHEMICAL ANALYSIS
TRITIUM COMPOUNDS
ISOTOPE APPLICATIONS
CATTLE
CEREBELLUM
CEREBRAL CORTEX
COMPARATIVE EVALUATIONS
NUCLEOSIDES
RATS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRUM
DOMESTIC ANIMALS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RIBOSIDES
RNA
RODENTS
RUMINANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550101 - Behavioral Biology- Tracer Techniques