Effect of soman on the cholinergic system in mouse brain
The effects of soman on levels of acetylcholine (ACh) and choline (Ch) and turnover rate of ACh have been studied in whole brain and brain regions (cerebellum, medulla-pons, midbrain, corpus striatum, hippocampus and cortex) of mice. Animals were injected with saline or a dose of soman up to 80..mu..g/kg, i.v. and were sacrificed by focussed microwave irradiation of the head. The tracer, /sup 3/H-Ch was injected (i.v.) 2 min prior to sacrifice and turnover rate of ACh was quantitated by using HPLC with electrochemical detection. A behaviorally effective dose of 80 ..mu..g/kg soman increased the levels of ACh significantly in whole brain (57.5%), corpus striatum (42.8%), hippocampus (24.1%) and cortex (43.1%). The levels of Ch were also increased in cerebellum (80.1%), midbrain (75.7%), corpus striatum (86.0%) and cortex (52.5%). The turnover rate of ACh was decreased in whole brain (53.8%), cerebellum (80.4%), medulla-pons (66.8%), midbrain (57.0%), corpus striatum (62.1%) and cortex (52.6%). The duration of these effects lasted more than 1 hr and the results indicate that the decrease in ACh turnover is not due necessarily to an increase in brain levels of ACh and/or Ch.
- Research Organization:
- Virginia Commonwealth Univ., Richmond
- OSTI ID:
- 7080669
- Report Number(s):
- CONF-8604222-; TRN: 87-009084
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
METABOLISM
TISSUE DISTRIBUTION
CHEMICAL WARFARE AGENTS
BIOLOGICAL EFFECTS
CHOLINE
MICROWAVE RADIATION
BRAIN
ENZYME INHIBITORS
MICE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIATIONS
RODENTS
VERTEBRATES
WEAPONS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques
560400 - Other Environmental Pollutant Effects