Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol
Aspen wood and wheat straw were pretreated by exposure to steam at elevated temperatures. Chemical analysis of the substrates revealed that steam explosion differentially decomposed the pentosan component while leaving the glucan portion relatively unchanged. The pretreated residues could be used as substrates for growth of Trichoderma reesei C30 and T. harzianum E58. The cellulase activities detected were in some cases three times as high as those found when Solka Floc was used as the substrate. Culture filtrates of T. harzianum E58 could efficiently hydrolyze the hemicellulose-rich water-soluble fractions. This material was fermented by Klebsiella pneumoniae with 0.4-0.5 g of 2,3-butanediol produced per gram of sugar utilized. Once the steam-exploded residues had been water and alkali extracted, the enzymatically hydrolyzed substrates were readily fermented by Saccharomyces cerevisiae or Zymononas mobilis with values as high as 2% (w/v) ethanol obtained from 5% steam-exploded wood fractions. 30 references, 2 figures, 8 tables.
- Research Organization:
- Forintek Canada Corp., Ottawa, Ontario
- OSTI ID:
- 7077505
- Report Number(s):
- CONF-830567-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 13; Conference: 5. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 10 May 1983
- Country of Publication:
- United States
- Language:
- English
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Enzymatic hydrolysis of cellulose and various pretreated wood fractions
Steam-explosion pretreatment of wood: effect of chip size, acid, moisture content and pressure drop
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BUTANEDIOLS
PRODUCTION
ETHANOL
STRAW
ENZYMATIC HYDROLYSIS
HEAT TREATMENTS
HYDROLYSIS
WOOD
ASPENS
CELLULASE
CHEMICAL COMPOSITION
ENZYME ACTIVITY
HEMICELLULOSE
KLEBSIELLA
SACCHAROMYCES CEREVISIAE
STEAM
SUBSTRATES
TRICHODERMA
TRICHODERMA VIRIDE
ZYMOMONAS MOBILIS
ALCOHOLS
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOLS
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHAROMYCES
SOLVOLYSIS
TREES
YEASTS
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