Assignment of the human RT6 gene to 11q13 by PCR screening of somatic cell hybrids and in situ hybridization

Journal Article · Mon Nov 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1484· OSTI ID:7075958

Koch-Nolte, F; Haag, F; Kuehl, M; Thiele, H G; Singh, S ^[1]; Van Heyningen, V ^[2]; Hoovers, J ^[3]; Grzeschik, K H ^[4]

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1. Univ. Hospital, Hamburg (Germany)
2. Medical Research Council, Edinburgh (United Kingdom)
3. Univ. of Amsterdam (Netherlands)
4. Univ. of Marburg (Germany)

RT6 is a T cell membrane protein that has attracted interest because a defect in RT6 expression is associated with susceptibility to autoimmune type I diabetes in DP-BB rats and NOD mice. Using PCR screening of human/rodent somatic cell hybrids and fluorescence in situ hybridization, the authors have determined that the gene for the human RT6 homologue is located at 11q13, centromeric to the gene for tyrosinase (TYR, albino locus) and telomeric to that for fibroblast growth factor 4 (FGF4). The data suggest that the human RT6 gene constitutes a new linkage group with TYR and the gene for olfactory marker protein (OMP) on 11q, which has a counterpart in mouse chromosome 7. Thus, in the human, the RT6 locus is dissociated from the hemoglobin [beta] chain locus (HBB) and its neighboring conserved linkage group at 11q15, in contrast to the mouse, in which RT6 shows a tighter linkage to Hbb than to Tyr. The results support the conclusion that there has been considerable intrachromosomal reshuffling of linked genes since the divergence of primates and rodents. 9 refs., 1 fig.

• OSTI ID: 7075958
• Journal Information: Genomics; (United States), Vol. 18:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIABETES MELLITUS
ETIOLOGY
HUMAN CHROMOSOMES
GENETIC MAPPING
LYMPHOCYTES
GENES
MEMBRANE PROTEINS
DNA HYBRIDIZATION
IMMUNE SYSTEM DISEASES
POLYMERASE CHAIN REACTION
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DISEASES
ENDOCRINE DISEASES
GENE AMPLIFICATION
HYBRIDIZATION
LEUKOCYTES
MAPPING
MATERIALS
METABOLIC DISEASES
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
550400* - Genetics
550900 - Pathology