Isolation and characterization of cDNA clones for human erythrocyte. beta. -spectrin
Spectrin is an important structural component of the membrane skeleton that underlies and supports the erythrocyte plasma membrane. It is composed of nonidentical ..cap alpha.. (M/sub r/ 240,000) and ..beta.. (M/sub r/ 220,000) subunits, each of which contains multiple homologous 106-amino acid segments. The authors report here the isolation and characterization of a human erythroid-specific ..beta..-spectrin cDNA clone that encodes parts of the ..beta..-9 through ..beta..-12 repeat segments. This cDNA was used as a hybridization probe to assign the ..beta..-spectrin gene to human chromosome 14 and to begin molecular analysis of the gene and its mRNA transcripts. RNA transfer blot analysis showed that the reticulocyte ..beta..-spectrin mRNA is 7.8 kilobases in length. Southern blot analysis of genomic DNA revealed the presence of restriction fragment length polymorphisms (RFLPs) within the ..beta..-spectrin gene locus. The isolation of human spectrin cDNA probes and the identification of closely linked RFLPs will facilitate analysis of mutant spectrin genes causing congenital hemolytic anemias associated with quantitative and qualitative spectrin abnormalities.
- Research Organization:
- Univ. of Alabama, Birmingham (USA)
- OSTI ID:
- 7075617
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:21
- Country of Publication:
- United States
- Language:
- English
Similar Records
Detection and characterization of a mouse. alpha. -spectrin cDNA clone by its expression in Escherichia coli
Cloning of cDNA encoding steroid 11. beta. -hydroxylase (P450c11)
Related Subjects
RECOMBINANT DNA
DNA SEQUENCING
ANTIBODIES
CELL MEMBRANES
ERYTHROCYTES
HYBRIDIZATION
MESSENGER-RNA
PHOSPHORUS 32
PROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA
ISOTOPES
LIGHT NUCLEI
MATERIALS
MEMBRANES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques