NADH electrochemical sensor coupled with dehydrogenase enzymes

Journal Article · Mon Jun 01 00:00:00 EDT 1992 · Analytical Letters; (United States)

DOI:https://doi.org/10.1080/00032719208020053· OSTI ID:7074525

Yamanaka, Hideko; Mascini, Marco ^[1]

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1. Epidemiologia e Chimica Analitica Ambientale, Firenze (Italy)

A graphite electrode assembled in a flow cell has shown to be a good detector for NADH. Current is linearly dependent on concentration in the range 10{sup {minus}7}-10{sup {minus}3} M without any mediator at the potential applied of 300 mV vs Ag/AgCl. Lactate and alcohol dehydrogenases were immobilized near to the electrode surface or in a reactor to obtain an NADH-based biosensor for lactate or ethanol. With lactate the authors succeeded to obtain a response only if the reactor was used and for alcohol a current proportional to the concentration was obtained either if the enzyme was immobilized in a membrane and placed near the electrode surface or when the enzyme was immobilized in a reactor form. By FIA procedures fast responses and recoveries were obtained, but with a short linear range.

• OSTI ID: 7074525
• Journal Information: Analytical Letters; (United States), Vol. 25:6; ISSN 0003-2719
• Country of Publication: United States
• Language: English

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