Cholinergic inhibition of adrenergic neurosecretion in the rabbit iris-ciliary body
The prejunctional effects of cholinergic agents on release of norepinephrine from sympathetic nerve endings were investigated in the isolated, superfused rabbit iris-ciliary body. Stimulation-evoked release of /sup 3/H-norepinephrine was inhibited by the cholinergic agonists methacholine, oxotremorine, muscarine, carbamylcholine and acetylcholine (plus eserine), but was unmodified by pilocarpine or nicotine. Agonist-induced inhibition was antagonized selectively by atropine, indicating a muscarinic response. Atropine alone markedly enhanced norepinephrine release, revealing considerable tonic activation of prejunctional cholinergic receptors in this system. Prejunctional inhibition by carbamylcholine was found to completely override the facilitative action of forskolin or 8-bromo-cyclic AMP on neurotransmitter release. Cholinergic and alpha 2-adrenergic effects on neurosecretion were non-additive, suggesting that the underlying receptors coexist at neurotransmitter release sites.
- Research Organization:
- Univ. of Louisville School of Medicine, KY (USA)
- OSTI ID:
- 7070141
- Journal Information:
- Invest. Ophthalmol. Visual Sci.; (United States), Vol. 29:4
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques