In vitro quantitation of lethal and physiologic effects of total body irradiation on stromal and hematopoietic stem cells in continuous bone marrow cultures from Rf mice

Journal Article · Thu Jul 01 00:00:00 EDT 1982 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(82)90063-3· OSTI ID:7066055

Greenberger, J S ^[1]; Eckner, R J; Otten, J A; Tennant, R W

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1. Havard Medical School, Boston, MA

The effects of in vivo total body irradiation (TBI) and interval from TBI to explant of marrow on: stromal cell proliferation in vitro; stromal cell support of hematopoiesis in continuous bone marrow culture; and generation of WEHI-3 growth factor (GF)-dependent lines of hematopoietic progenitor cells were evaluated. Explant of marrow at 2, 4, 5, or 6 months after single fraction TBI (300-800 rad) was associated with decreased longevity of hemopoiesis and a decrease in the proliferative capacity of fibroblastic adherent-stromal colony forming cells (CFUf) as measured by colony size at 14 days and number of colonies per 10/sup 6/ cells plated. In contrast, explant of marrow 8 to 24 months after TBI produced cultures with longevity that was indistinguishable from age-matched control cultures (19-24 weeks). Marrow from irradiated first and second generation recipients of serially transferred marrow demonstrated a similar 7-month in vivo recovery period; however, the plateau maximum duration of hemopoiesis did not return to control levels. Purified stromal cell cultures were prepared by corticosteroid-deprivation of explanted marrow for 28 days and were then engrafted in vitro with marrow from C57BL/6J or RfM/UN mice that had been irradiated 1 month previously. Hemopoiesis in these cultures was restored, and they produced GM-CFUc and granulocytes for 15-24 weeks. Thus, healthy stroma supported growth of recently irradiated hemopoietic cells in vitro. Indirect effects of x-irradiation on hemopoietic stem cells through damage and repair in the stromal cell compartment can be effectively studied with the present bone marrow culture system. (JMT)

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 7066055
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 8:7
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MICE
WHOLE-BODY IRRADIATION
STEM CELLS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
BLOOD FORMATION
BONE MARROW
CELL PROLIFERATION
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
X RADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
IRRADIATION
MAMMALS
ORGANS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
560121 - Radiation Effects on Cells- External Source- (-1987)