A strategy for fine-structure functional analysis of a 6- to 11-centimorgan region of mouse chromosome 7 by high-efficiency mutagenesis

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.3.896· OSTI ID:7063855

Rinchik, E M; Carpenter, D A; Selby, P B ^[1]

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1. Oak Ridge National Laboratory, TN (USA)

A refined functional map of a 6- to 11-centimorgan region surrounding the albino (c) locus in mouse chromosome 7 is being generated by N-ethyl-N-nitrosourea (EtNU) saturation mutagenesis of stem-cell spermatogonia. In the first phase of an experiment that will eventually test at least 3,000 gametes, the authors screened 972 mutagenized gametes for the induction of both lethal and visible mutations with a two-cross breeding protocol. Thirteen mutations mapping within the limits of a segment corresponding to the cytologically visible Df(c Mod-2 sh-1){sup 26DVT} deletion were recovered. They represented three phenotypic groups: prenatal lethality (six mutations); a fitness/runting syndrome (three mutations, provisionally designated as fit variants); and a neurological/balance-defect abnormality (four mutations). Complementation analysis provided evidence for a true repeat mutation at the sh-1 (shaker-1) locus (for the neurological mutations) and another at the here defined fit-1 (fitness-1) locus. The recovery of the repeat mutations suggests that the EtNU-induced mutation rate, estimated from specific-locus tests, should make it possible to achieve saturation mutagenesis of a chromosomal region. This experiment is providing basic logistical and statistical information on which to base strategies for expanding the functional map of larger segments of the mouse genome by experimental mutagenesis.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7063855
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:3; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
MOLECULAR STRUCTURE
GENE MUTATIONS
GENETIC MAPPING
NITROSOUREAS
GENETIC EFFECTS
LETHAL MUTATIONS
MICE
MUTAGEN SCREENING
MUTAGENESIS
SPERMATOGONIA
STEM CELLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
GAMETES
GERM CELLS
MAMMALS
MAPPING
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RODENTS
SCREENING
SOMATIC CELLS
VERTEBRATES
550400* - Genetics