Aerobic vinyl chloride metabolism in Mycobacterium aurum L1

Journal Article · Wed Apr 01 00:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7044725

Hartmans, S; Bont, J A.M. de ^[1]

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1. Wageningen Agricultural Univ. (Netherlands)

Mycobacterium aurum L1, capable of growth on vinyl chloride as a sole carbon and energy source, was previously isolated from soil contaminated with vinyl chloride. The initial step in vinyl chloride metabolism in strain L1 is catalyzed by alkene monooxygenase, transforming vinyl chloride into the reactive epoxide chlorooxirane. The enzyme responsible for chlorooxirane degradation appeared to be very unstable and thus hampered the characterization of the second step in vinyl chloride metabolism. Dichloroethenes are also oxidized by vinyl chloride-grown cells of strain L1, but they are not utilized as growth substrates. Three additional bacterial strains which utilize vinyl chloride as a sole carbon and energy source were isolated from environments with no known vinyl chloride contamination. The three new isolates were similar to strain L1 and were also identified as Mycobacterium aurum.

• OSTI ID: 7044725
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 58:4; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
METABOLISM
VINYL CHLORIDE
BIODEGRADATION
CONTAMINATION
DECONTAMINATION
METABOLITES
OXYGENASES
SOILS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CLEANING
DECOMPOSITION
ENZYMES
HALOGENATED ALIPHATIC HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PROTEINS
560300* - Chemicals Metabolism & Toxicology