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Title: Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures

Abstract

Several Candida albicans morphological mutants were obtained by a procedure based on a combined treatment with nitrous acid plus UV irradiation and a double-enrichment step to increase the proportion of mutants growing as long filamentous structures. Altered cell morphogenesis in these mutants correlated with an altered colonial phenotype. Two of these mutants, C. albicans NEL102 and NEL103, were selected and characterized. Mutant blastoconidia initiated budding but eventually gave rise to filamentous hypha-type formations. These filaments were long and septate, and they branched very regularly at positions near septa. Calcofluor white (which is known to bind chitin-rich areas) stained septa, branching zones, and filament tips very intensely, as observed under the fluorescence microscope. Wild-type hybrids were obtained by fusing protoplasts of strain NEL102 with B14, another morphological mutant previously described as being permanently pseudomycelial, indicating that genetic determinants responsible for the two altered phenotypes are different. The mutants characterized in this work seemed to sequentially express the morphogenic characteristics of C. albicans, from blastoconidia to hyphae, in the absence of any inducer. Further characterization of these strains could be relevant to gain understanding of the genetic control of dimorphism in this species.

Authors:
; ;  [1]
  1. Universidad Complutense, Madrid (Spain)
Publication Date:
OSTI Identifier:
7043277
Resource Type:
Journal Article
Journal Name:
Journal of Bacteriology; (USA)
Additional Journal Information:
Journal Volume: 172:5; Journal ID: ISSN 0021-9193
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CANDIDA; GENETIC RADIATION EFFECTS; GENE MUTATIONS; RADIOINDUCTION; FLUORESCENCE; MICROSCOPY; MITOSIS; NITROUS ACID; PHENOTYPE; RADIATION INDUCED MUTANTS; ULTRAVIOLET RADIATION; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CELL DIVISION; ELECTROMAGNETIC RADIATION; EUMYCOTA; FUNGI; GENETIC EFFECTS; HYDROGEN COMPOUNDS; INORGANIC ACIDS; LUMINESCENCE; MICROORGANISMS; MUTANTS; MUTATIONS; PLANTS; RADIATION EFFECTS; RADIATIONS; YEASTS; 560130* - Radiation Effects on Microorganisms

Citation Formats

Gil, C, Pomes, R, and Nombela, C. Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures. United States: N. p., 1990. Web.
Gil, C, Pomes, R, & Nombela, C. Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures. United States.
Gil, C, Pomes, R, and Nombela, C. 1990. "Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures". United States.
@article{osti_7043277,
title = {Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures},
author = {Gil, C and Pomes, R and Nombela, C},
abstractNote = {Several Candida albicans morphological mutants were obtained by a procedure based on a combined treatment with nitrous acid plus UV irradiation and a double-enrichment step to increase the proportion of mutants growing as long filamentous structures. Altered cell morphogenesis in these mutants correlated with an altered colonial phenotype. Two of these mutants, C. albicans NEL102 and NEL103, were selected and characterized. Mutant blastoconidia initiated budding but eventually gave rise to filamentous hypha-type formations. These filaments were long and septate, and they branched very regularly at positions near septa. Calcofluor white (which is known to bind chitin-rich areas) stained septa, branching zones, and filament tips very intensely, as observed under the fluorescence microscope. Wild-type hybrids were obtained by fusing protoplasts of strain NEL102 with B14, another morphological mutant previously described as being permanently pseudomycelial, indicating that genetic determinants responsible for the two altered phenotypes are different. The mutants characterized in this work seemed to sequentially express the morphogenic characteristics of C. albicans, from blastoconidia to hyphae, in the absence of any inducer. Further characterization of these strains could be relevant to gain understanding of the genetic control of dimorphism in this species.},
doi = {},
url = {https://www.osti.gov/biblio/7043277}, journal = {Journal of Bacteriology; (USA)},
issn = {0021-9193},
number = ,
volume = 172:5,
place = {United States},
year = {Tue May 01 00:00:00 EDT 1990},
month = {Tue May 01 00:00:00 EDT 1990}
}