Absorption and lymphatic transport of exogenous and endogenous arachidonic and linoleic acid in the rat

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:7037745

Nilsson, A; Landin, B; Jensen, E; Akesson, B

(/sup 3/H)Arachidonic (20:4) and (/sup 14/C)linoleic acid (18:2) were fed to thoracic duct-cannulated rats in test meals of either tracers alone, cream, Intralipid, pure arachidonic acid, or pure linoleic acid. Less (/sup 3/H)20:4 than (/sup 14/C)18:2 was recovered in chyle during the first 5 h. After cream feeding, the proportion of radioactivity found in phospholipids was high and increased during the first 3 h. After the meal 61 +/- 6% of the /sup 3/H and 57 +/- 10% of the /sup 14/C was in phosphatidylcholine, and 11 +/- 3% of the /sup 3/H and 3.0 +/- 4% of the /sup 14/C was in phosphatidylethanolamine. Changing the fat vehicle to Intralipid or pure 18:2 decreased the proportion of label in the phospholipds and increased the /sup 3/H and /sup 14/C radioactivity in the triacylglycerol fraction, the distribution of /sup 14/C radioactivity in the triacylglycerol fraction, the distribution of /sup 14/C being influenced more than that of /sup 3/H. After feeding the tracers in 200 ..mu..l of pure 20:4, >90% of both isotopes was in triacylglycerol. During fasting, triacylglycerol transported 56% (0.7 ..mu..mol/h), phosphatidylethanolamine transported 10% (0.1 ..mu..mol/h) of the 20:4 mass. After cream or Intralipid feeding, the output of 20:4-containing phosphatidylcholine and phosphatidylethanolamine increased 2.1- to 2.8-fold, whereas the transport of 20:4 with triacylglycerol remained constant. Phospholipids thus became the predominant transport form for 20:4. After feeding 200 ..mu..l of 20:4, the intestine produced, however, 20:4-rich triacylglycerols that transported 80% of the chyle 20:4.

• Research Organization: Univ. of Lund (Sweden)
• OSTI ID: 7037745
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LABELLED COMPOUNDS
INTESTINAL ABSORPTION
MASS TRANSFER
MONOCARBOXYLIC ACIDS
ARACHIDONIC ACID
CARBON 14 COMPOUNDS
DIET
LINOLEIC ACID
LYMPHATIC SYSTEM
PHOSPHOLIPIDS
PHYSIOLOGY
RATS
TRITIUM COMPOUNDS
ABSORPTION
ANIMALS
CARBOXYLIC ACIDS
ESTERS
LIPIDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
RODENTS
UPTAKE
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques