Production of monoclonal antibody recognizing a subpopulation of human B lymphocytes producing B cell growth factor (BCGF)

Journal Article · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7034997

Witzel, N; Ambrus, Jr, J L; Jurgensen, C H; Mostowski, H; Fauci, A S

Several laboratories have recently reported production of B cell growth factors (BCGF) by a variety of human B cell lines. The authors have described production of BCGF by clones of B cell lymphoma lines and by normal Staphylococcus aureus Cowan I-activated B cells. To further evaluate whether BCGF production was the function of particular subpopulations of B cells, they immunized mice with the BCGF producing line Namalva and then developed a panel of hybridomas. Monoclonal antibodies were screened for binding to Namalva and the absence of binding to a non-BCGF producing B cell line. One monoclonal antibody, NN4, which met these criteria was also noted by fluorescence-activated cell sorter analysis to stain clones from a B cell lymphoma line producing BCGF but not clones from the same line which do not produce BCGF. The monoclonal antibody did not stain T cells or monocytes but stained 1-5% of normal human B lymphocytes from peripheral blood or tonsils. Binding of /sup 125/I-labeled NN4 to NN4/sup +/ B cells was not affected by the presence of BCGF. Therefore, NN4 recognizes a unique antigen present on B cell lines which produces BCGF; studies are in progress to determine the significance of this antigen on normal B cells.

• Research Organization: National Health Institutes, Bethesda, MD
• OSTI ID: 7034997
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

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HYBRIDOMAS
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550201* - Biochemistry- Tracer Techniques