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Title: Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop

Abstract

To identify the mechanism of Cl{sup {minus}} transport across the thin ascending limb of Henle's loop (TAL), the authors examined effects of anion transport inhibitors and ionic substitution in the isolated segments of hamsters using the in vitro microperfusion technique. 4,4{prime}-diisothiocyanostilbene-2,2{prime}-disulfonic acid (DIDS) at 10{sup {minus}3} M changed the NaCl diffusion voltage (V{sub t}) to the orientation that corresponds to the decrease in the Cl{sup {minus}}-Na{sup +} permeability ratio when it was added either to the bath or to the lumen. DIDS, added to the bath or to the lumen decreased the lumen-to-bath flux coefficient for {sup 36}Cl, whereas it had little effect on the flux coefficient for {sup 22}Na. The inhibitory effect of phloretin was rapid and reversible. Phloridzin was ineffective. From these observations, they conclude that Cl{sup {minus}} transport across the TAL is distinct from Na{sup +} and is not coupled with Na{sup +}, K{sup +}, or HCO{sup {minus}}{sub 3}.

Authors:
; ;  [1]
  1. National Cardiovascular Center Research Institute, Osaka (Japan)
Publication Date:
OSTI Identifier:
7027254
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 253:6; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHLORINE 36; MEMBRANE TRANSPORT; ENZYME INHIBITORS; BIOCHEMISTRY; RENAL CLEARANCE; BIOCHEMICAL REACTION KINETICS; SODIUM 22; ANIONS; DOSE-RESPONSE RELATIONSHIPS; HAMSTERS; KIDNEYS; MEMBRANE PROTEINS; OSMOSIS; POTASSIUM CHLORIDES; SODIUM CHLORIDES; SULFONIC ACIDS; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CHARGED PARTICLES; CHEMISTRY; CHLORIDES; CHLORINE COMPOUNDS; CHLORINE ISOTOPES; CLEARANCE; DIFFUSION; ELECTRON CAPTURE RADIOISOTOPES; EXCRETION; HALIDES; HALOGEN COMPOUNDS; IONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; KINETICS; LIGHT NUCLEI; MAMMALS; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; POTASSIUM COMPOUNDS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SODIUM COMPOUNDS; SODIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Kondo, Yoshiaki, Yoshitomi, Koji, and Imai, Masashi. Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop. United States: N. p., 1987. Web.
Kondo, Yoshiaki, Yoshitomi, Koji, & Imai, Masashi. Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop. United States.
Kondo, Yoshiaki, Yoshitomi, Koji, and Imai, Masashi. 1987. "Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop". United States.
@article{osti_7027254,
title = {Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop},
author = {Kondo, Yoshiaki and Yoshitomi, Koji and Imai, Masashi},
abstractNote = {To identify the mechanism of Cl{sup {minus}} transport across the thin ascending limb of Henle's loop (TAL), the authors examined effects of anion transport inhibitors and ionic substitution in the isolated segments of hamsters using the in vitro microperfusion technique. 4,4{prime}-diisothiocyanostilbene-2,2{prime}-disulfonic acid (DIDS) at 10{sup {minus}3} M changed the NaCl diffusion voltage (V{sub t}) to the orientation that corresponds to the decrease in the Cl{sup {minus}}-Na{sup +} permeability ratio when it was added either to the bath or to the lumen. DIDS, added to the bath or to the lumen decreased the lumen-to-bath flux coefficient for {sup 36}Cl, whereas it had little effect on the flux coefficient for {sup 22}Na. The inhibitory effect of phloretin was rapid and reversible. Phloridzin was ineffective. From these observations, they conclude that Cl{sup {minus}} transport across the TAL is distinct from Na{sup +} and is not coupled with Na{sup +}, K{sup +}, or HCO{sup {minus}}{sub 3}.},
doi = {},
url = {https://www.osti.gov/biblio/7027254}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 253:6,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 1987},
month = {Tue Dec 01 00:00:00 EST 1987}
}