Pharmacological characterization of. cap alpha. /sub 2/-adrenoceptor heterogeneity
Utilizing the techniques of radioligand binding assay and quantitative autoradiography, evidence is provided for the existence of a heterogeneous population of ..cap alpha../sub 2/-adrenoceptors, which is present in central as well as peripheral tissues, and across two species. In rat brain, the autoradiographic distributions of binding sites labeled by two reportedly selective ..cap alpha../sub 2/-adrenoceptor antagonists, (/sup 3/H) rauwolscine and (/sup 3/H) idazoxan, differed markedly throughout the neuraxis. Whereas (/sup 3/H)idazoxan labeling appeared over brain regions receiving noradrenergic innervations, (/sup 3/H)rauwolscine binding sites were localized most densely in several areas corresponding to dopaminergic terminal fields. In areas labeled by (/sup 3/H)rauwolscine, the maximal binding densities for (/sup 3/H)idazoxan labeling were consistently greater than those for (/sup 3/H)rauwolscine sites. The autoradiographic distributions of (/sup 3/H)idazoxan and (/sup 3/H)-rauwolscine binding sites throughout monkey brain were very similar to those observed in rat brain, suggesting that the R/sub I/ and R/sub S/ sites characterized in the rodent central nervous system may also exist in primate brain.
- Research Organization:
- California Univ., Irvine (USA)
- OSTI ID:
- 7027159
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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RATS
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
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CENTRAL NERVOUS SYSTEM
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550201* - Biochemistry- Tracer Techniques