Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene

Journal Article · Sun May 01 00:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.5.1985· OSTI ID:7027018

Feinbaum, R L; Ausubel, F M

The authors cloned an Arabiodpsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiment.

• Research Organization: Dept. of Genetics, Harvard Medical School, Boston, MA (US)
• OSTI ID: 7027018
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIGASES
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENES
ARABIDOPSIS
EXPERIMENTAL DATA
HYBRIDIZATION
MESSENGER-RNA
TRANSCRIPTION
CLONING
DATA
ENZYMES
INFORMATION
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
RNA
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry