New fluorescence reactions in DNA cytochemistry. 2. Microscopic and spectroscopic studies on fluorescent aluminum complexes

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Analytical and Quantitative Cytology and Histology; (USA)

OSTI ID:7026988

Del Castillo, P; Llorente, A R; Gomez, A; Gosalvez, J; Goyanes, V J; Stockert, J C ^[1]

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1. Autonomous Univ., Madrid (Spain)

Metal-dye complexes are widely applied in light microscopic techniques for chromatin staining (e.g., hematoxylin and carmine), but fluorescent complexes between phosphate-binding cations and suitable ligands have been little used. Preformed and postformed Al complexes with different anionic dyes induced strong and selective fluorescence reactions in nuclei from chicken blood smears, frozen sections, paraffin-embedded sections and Epon-embedded sections of mouse and rat tissues, mitotic chromosomes, meiotic chromosomes and kinetoplasts of Trypanosoma cruzi epimastigotes. The DNA-dependent fluorescence of these structures showed a very low fading rate. The emission colors were related to the ligand. The most suitable compounds for forming fluorescent Al chelates were 8-hydroxyquinoline, morin, nuclear fast red and purpurin. Staining with diluted carmine solutions and InCl3 mordanting, followed by 8-hydroxyquinoline, also induced chromatin fluorescence. After treating isolated mouse chromosomes with the preformed complex Al-nuclear fast red, x-ray microanalysis indicated a P:Al:dye binding ratio of about 40:15:1. The selectivity, stability and easy formation of these fluorescent Al complexes are obvious advantages for their use as new cytochemical probes in cytologic studies.

• OSTI ID: 7026988
• Journal Information: Analytical and Quantitative Cytology and Histology; (USA), Vol. 12:1; ISSN 0884-6812
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALUMINIUM
FLUORESCENCE
CHROMOSOMES
ELECTRON MICROPROBE ANALYSIS
DNA
CYTOLOGICAL TECHNIQUES
CELL NUCLEI
CHELATING AGENTS
CHICKENS
CHROMATIN
COMPLEXES
DYES
MICE
RATS
ANIMALS
BIRDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
ELEMENTS
FOWL
LUMINESCENCE
MAMMALS
METALS
MICROANALYSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RODENTS
VERTEBRATES
550301* - Cytology- Tracer Techniques