Fate of oleate in the colon of the rat

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Journal of Laboratory and Clinical Medicine; (USA)

OSTI ID:7026948

Segal, L; Kneip, J; Levitt, M D ^[1]

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1. Veterans Administration Medical Center, Minneapolis, MN (USA)

To study the fate of oleate in the colon, oleate labeled with carbon 14 was instilled into the cecum of four rats through chronically implanted cecal cannulas. Fecal recovery of {sup 14}C and {sup 14}CO{sub 2} excretion were measured over a 3-day period. A mean of only 57% +/- 6% of the dose of ({sup 14}C(U))oleate was recovered as {sup 14}C in fecal lipid. About 8% was recovered in the aqueous phase of feces and 4% was recovered as {sup 14}CO{sub 2}. Occlusion of the terminal ileum did not diminish {sup 14}CO{sub 2} excretion, excluding ileal reflux with small bowel absorption. Studies in two germ-free rats showed no conversion of ({sup 14}C(U))oleate to fecal water-soluble compounds, indicating that ({sup 14}C)oleate is converted into water-soluble compounds by bacterial metabolism. The metabolism of ({sup 14}C)oleate to {sup 14}CO{sub 2} was also observed in germ-free rats, indicating oxidation in the colon or other host tissues. We conclude that the colonic absorption of lipid or lipid metabolites plus conversion to water-soluble fecal compounds or volatile metabolites results in a fecal fat measurement that appreciably overestimates small bowel absorption of lipid.

• OSTI ID: 7026948
• Journal Information: Journal of Laboratory and Clinical Medicine; (USA), Vol. 115:2; ISSN 0022-2143
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OLEIC ACID
METABOLISM
CARBON 14 COMPOUNDS
CARBON DIOXIDE
FECES
INTESTINAL ABSORPTION
LARGE INTESTINE
METABOLITES
RATS
TRACER TECHNIQUES
ABSORPTION
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RODENTS
UPTAKE
VERTEBRATES
WASTES
550501* - Metabolism- Tracer Techniques