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Title: Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. [Avena sativa L. ; Beta vulgaris]

Abstract

Acridine orange altered the response to anions of both ATP and inorganic pyrophosphate-dependent pH gradient formation in tonoplast vesicles isolated from oat (Avena sativa L.) roots and red beet (Beta vulgaris L.) storage tissue. When used as a fluorescent pH probe in the presence of I{sup {minus}}, ClO{sub 3}{sup {minus}}, NO{sub 3}{sup {minus}}, Br{sup {minus}}, or SCN{sup {minus}}, acridine orange reported lower pH gradients than either quinacrine or ({sup 14}C)methylamine. Acridine orange, but not quinacrine, reduced ({sup 14}C)methylamine accumulation when NO{sub 3}{sup {minus}} was present indicating that the effect was due to a real decrease in the size of the pH gradient, not a misreporting of the gradient by acridine orange. Other experiments indicated that acridine orange and NO{sub 3}{sup {minus}} increased the rate of pH gradient collapse both in tonoplast vesicles and in liposomes of phosphatidylcholine and that the effect in tonoplast vesicles was greater at 24{degree}C than at 12{degree}C. It is suggested that acridine orange and certain anions increase the permeability of membranes to H{sup +}, possibly because protonated acridine orange and the anions form a lipophilic ion pair within the vesicle which diffuses across the membrane thus discharging the pH gradient.

Authors:
;  [1]
  1. AFRC Institute of Arable Crops Research, Harpenden (England)
Publication Date:
OSTI Identifier:
7025807
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 86:4; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; PERMEABILITY; LIPOSOMES; BIOCHEMICAL REACTION KINETICS; PHYSIOLOGY; ACRIDINE ORANGE; ANIONS; BEETS; BROMIDES; CARBON 14 COMPOUNDS; CELL FLOW SYSTEMS; CHLORATES; IODIDES; LECITHINS; NITRATES; OATS; PH VALUE; RESPONSE MODIFYING FACTORS; ROOTS; TEMPERATURE DEPENDENCE; THIOCYANATES; TRACER TECHNIQUES; ACRIDINES; AMINES; ANTITHYROID DRUGS; AROMATICS; AZAARENES; AZINES; BROMINE COMPOUNDS; CARBONIC ACID DERIVATIVES; CELL CONSTITUENTS; CEREALS; CHARGED PARTICLES; CHLORINE COMPOUNDS; DRUGS; DYES; ESTERS; FOOD; GRASS; HALIDES; HALOGEN COMPOUNDS; HETEROCYCLIC COMPOUNDS; HORMONE ANTAGONISTS; IODINE COMPOUNDS; IONS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; LILIOPSIDA; LIPIDS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MEMBRANES; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANOIDS; OXYGEN COMPOUNDS; PHOSPHOLIPIDS; PLANTS; PYRIDINES; REACTION KINETICS; VEGETABLES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Pope, A J, and Leigh, R A. Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. [Avena sativa L. ; Beta vulgaris]. United States: N. p., 1988. Web. doi:10.1104/pp.86.4.1315.
Pope, A J, & Leigh, R A. Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. [Avena sativa L. ; Beta vulgaris]. United States. https://doi.org/10.1104/pp.86.4.1315
Pope, A J, and Leigh, R A. 1988. "Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. [Avena sativa L. ; Beta vulgaris]". United States. https://doi.org/10.1104/pp.86.4.1315.
@article{osti_7025807,
title = {Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. [Avena sativa L. ; Beta vulgaris]},
author = {Pope, A J and Leigh, R A},
abstractNote = {Acridine orange altered the response to anions of both ATP and inorganic pyrophosphate-dependent pH gradient formation in tonoplast vesicles isolated from oat (Avena sativa L.) roots and red beet (Beta vulgaris L.) storage tissue. When used as a fluorescent pH probe in the presence of I{sup {minus}}, ClO{sub 3}{sup {minus}}, NO{sub 3}{sup {minus}}, Br{sup {minus}}, or SCN{sup {minus}}, acridine orange reported lower pH gradients than either quinacrine or ({sup 14}C)methylamine. Acridine orange, but not quinacrine, reduced ({sup 14}C)methylamine accumulation when NO{sub 3}{sup {minus}} was present indicating that the effect was due to a real decrease in the size of the pH gradient, not a misreporting of the gradient by acridine orange. Other experiments indicated that acridine orange and NO{sub 3}{sup {minus}} increased the rate of pH gradient collapse both in tonoplast vesicles and in liposomes of phosphatidylcholine and that the effect in tonoplast vesicles was greater at 24{degree}C than at 12{degree}C. It is suggested that acridine orange and certain anions increase the permeability of membranes to H{sup +}, possibly because protonated acridine orange and the anions form a lipophilic ion pair within the vesicle which diffuses across the membrane thus discharging the pH gradient.},
doi = {10.1104/pp.86.4.1315},
url = {https://www.osti.gov/biblio/7025807}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 86:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1988},
month = {Fri Apr 01 00:00:00 EST 1988}
}