Effects of ethanol and hyperosmotic perfusates on albumin synthesis and release
Sucrose and ethanol inhibit albumin synthesis; sucrose via an osmotic mechanism and ethanol during its metabolism. The present study was undertaken to compare the effects of both of these agents on albumin synthesis and secretion, and to see if ethanol inhibition could be related to an osmotic effect. Male, fed rabbits served as liver donors in all studies. There were a total of 35 studies: 13 control; 10 ethanol (39 to 52 mM); 4 cycloheximide (0.5 mM), and 8 sucrose (1%). Plasma volume was measured with /sup 125/I-albumin (human) and extracellular volume measured with either /sup 99m/Tc diethylenetriamine pentaacetic acid or (/sup 14/C)sucrose. During perfusion, rabbit albumin content in the perfusate was measured immunologically every 15 to 30 min for 225 min. Interstitial albumin efflux was measured by the rate of appearance in the perfusate of /sup 125/I-albumin given to 10 other rabbits 3 days prior to hepatic removal and perfusion. During the initial 75 min of perfusion, 74% of the in vivo equilibrated exchangeable /sup 125/I-albumin appeared in the perfusate, and during this period the rabbit albumin that entered the perfusate was taken to represent efflux from the interstitial volume plus synthesis. Rabbit albumin appearing in the perfusate during the later period of 150 min was taken to represent mainly synthesis and was used to calculate the amount of albumin that would be synthesized in 75 min. The difference between these two values would be hepatic interstitial albumin appearing in the perfusate.
- Research Organization:
- Veterans Administration Medical Center, New York, NY
- OSTI ID:
- 7025465
- Journal Information:
- Hepatol.; (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOSYNTHESIS
ETHANOL
BIOLOGICAL EFFECTS
SACCHAROSE
ACETIC ACID
CARBON 14 COMPOUNDS
CYCLOHEXIMIDE
DTPA
EXTRACELLULAR SPACE
IODINE 125
ISOMERIC NUCLEI
LIVER
RABBITS
TECHNETIUM
TECHNETIUM 99
TRACER TECHNIQUES
ALCOHOLS
AMINO ACIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISACCHARIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
FUNGICIDES
GLANDS
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
METALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SACCHARIDES
SPACE
SYNTHESIS
TECHNETIUM ISOTOPES
TRANSITION ELEMENTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques