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Title: Rapid and convenient preparation of (4-/sup 3/H)NADP and stereospecifically tritiated NADP/sup 3/H

Abstract

The enzymatic preparation and chromatographic purification of (4-/sup 3/H)NADP and NADPH stereospecifically labeled with /sup 3/H on either the A or B faces at position 4 have been simplified. Commercially available (1-/sup 3/H)glucose was used as a starting material for the sequential synthesis of (4B-/sup 3/H)NADPH, (4-/sup 3/H)NADP, and (4A-/sup 3/H)NADPH. These products were rapidly purified by step elution of DEAE-cellulose minicolumns so that (4B-/sup 3/H)NADPH was produced and purified from (1-/sup 3/H)glucose in 2 h, (4-/sup 3/H)NADP in 5 h, and (4A-/sup 3/H)NADPH in 8 h. Yields of these products were 65 to 88% starting with (1-/sup 3/H)glucose. Analysis of the products by high-performance liquid chromatography indicated radiochemical purities of 82-95% for these compounds and specific activities equivalent to that of the starting material (10-15 Ci/mmol). 15 references.

Authors:
; ;
Publication Date:
Research Org.:
Childrens Hospital of Los Angeles, CA
OSTI Identifier:
7025085
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 138:1
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; COENZYMES; STEREOCHEMISTRY; SYNTHESIS; GLUCOSE; LABELLED COMPOUNDS; LIQUID COLUMN CHROMATOGRAPHY; PURIFICATION; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALDEHYDES; CARBOHYDRATES; CHROMATOGRAPHY; HEXOSES; ISOTOPE APPLICATIONS; MONOSACCHARIDES; ORGANIC COMPOUNDS; SACCHARIDES; SEPARATION PROCESSES; 400102* - Chemical & Spectral Procedures

Citation Formats

Moran, R G, Sartori, P, and Reich, V. Rapid and convenient preparation of (4-/sup 3/H)NADP and stereospecifically tritiated NADP/sup 3/H. United States: N. p., 1984. Web. doi:10.1016/0003-2697(84)90788-7.
Moran, R G, Sartori, P, & Reich, V. Rapid and convenient preparation of (4-/sup 3/H)NADP and stereospecifically tritiated NADP/sup 3/H. United States. https://doi.org/10.1016/0003-2697(84)90788-7
Moran, R G, Sartori, P, and Reich, V. 1984. "Rapid and convenient preparation of (4-/sup 3/H)NADP and stereospecifically tritiated NADP/sup 3/H". United States. https://doi.org/10.1016/0003-2697(84)90788-7.
@article{osti_7025085,
title = {Rapid and convenient preparation of (4-/sup 3/H)NADP and stereospecifically tritiated NADP/sup 3/H},
author = {Moran, R G and Sartori, P and Reich, V},
abstractNote = {The enzymatic preparation and chromatographic purification of (4-/sup 3/H)NADP and NADPH stereospecifically labeled with /sup 3/H on either the A or B faces at position 4 have been simplified. Commercially available (1-/sup 3/H)glucose was used as a starting material for the sequential synthesis of (4B-/sup 3/H)NADPH, (4-/sup 3/H)NADP, and (4A-/sup 3/H)NADPH. These products were rapidly purified by step elution of DEAE-cellulose minicolumns so that (4B-/sup 3/H)NADPH was produced and purified from (1-/sup 3/H)glucose in 2 h, (4-/sup 3/H)NADP in 5 h, and (4A-/sup 3/H)NADPH in 8 h. Yields of these products were 65 to 88% starting with (1-/sup 3/H)glucose. Analysis of the products by high-performance liquid chromatography indicated radiochemical purities of 82-95% for these compounds and specific activities equivalent to that of the starting material (10-15 Ci/mmol). 15 references.},
doi = {10.1016/0003-2697(84)90788-7},
url = {https://www.osti.gov/biblio/7025085}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 138:1,
place = {United States},
year = {Sun Apr 01 00:00:00 EST 1984},
month = {Sun Apr 01 00:00:00 EST 1984}
}