Specific reduction of N,N-dimethylnitrosamine mutagenicity in Drosophila melanogaster by dimethyl sulfoxide

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Environ. Mol. Mutagen.; (United States)

OSTI ID:7024589

Brodberg, R K; Mitchell, M J; Smith, S L; Woodruff, R C

Dimethyl sulfoxide (DMSO) used as a solvent has been observed to complicate mutagenicity screens by interacting with tested chemicals to yield false positive or negatives. The authors have used DMSO as a solvent in the Drosophila melanogaster recessive sex-linked lethal mutation assay and find that it reduces, but does not abolish, the detectable mutagenicity of N,N-dimethylnitrosamine (DMN). Its use as a solvent with procarbazine, another promutagen, shows no effect on mutagenicity in Drosophila. DMSO does not exhibit a general inhibitory action on microsome activity when ecdysone 20-monooxygenase activity is used as a measure of cytochrome P-450 activity. They were unable to detect the low DMN demethylase activity in the strain used. Hence, the inhibitory effect of DMSO in Drosophila at both the physiological and biological level appears to be limited and not general in action. Because DMN and DMSO are similar in structure, it is possible that DMSO is interacting with a DMN demethylase in Drosophila. This might lead to a reduction in the conversion of DMN to a mutagen. Consequently, from the results of this study and others DMSO should be used cautiously as a solvent in Drosophila mutagen screening.

• Research Organization: Univ. of California, Davis (USA)
• OSTI ID: 7024589
• Journal Information: Environ. Mol. Mutagen.; (United States), Vol. 10:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DMSO
BIOCHEMISTRY
BIOLOGICAL EFFECTS
NITROSAMINES
MUTAGEN SCREENING
DROSOPHILA
MICROSOMES
MUTAGENS
OXYGENASES
AMINES
ANIMALS
ARTHROPODS
CELL CONSTITUENTS
CHEMISTRY
DIPTERA
ENZYMES
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
SCREENING
SULFOXIDES
560300* - Chemicals Metabolism & Toxicology