Effect of zinc deficiency on NADPH and cytochrome P-450 dependent active oxygen generation in rat lung and liver

Journal Article · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7024519

Hammermueller, J D; Bray, T M; Bettger, W J

The cyt. P-450 system and cyt. P-450 reductase are involved in the generation of active oxygen species such as H/sub 2/O/sub 2/. The objective of this study was to investigate the effect of short term, severe, dietary zinc deficiency in rats on the formation of active oxygen in vitro. Weanling male Wistar rats were fed egg white-based diets containing less than 1 ppm Zn (ZnD). Controls were fed ad libitum (ZnAl) or pair-fed (ZnPF) a diet containing 100 ppm Zn. After 3 weeks lung and liver microsomes were assayed for H/sub 2/O/sub 2/ production (pmol H/sub 2/O/sub 2//mg protein/min) and cyt. P-450 reductase activity (nmol cyt. C reduced/mg protein/min). For the measurement of H/sub 2/O/sub 2/ production exogenous substrate (aminopyrine) and NADPH (cofactor) were provided to drive the cyt. P-450 system and NaN/sub 3/ was used to inhibit catalase. The results showed a significant effect of dietary Zn on NADPH and cyt. P-450 dependent active oxygen generation and support the hypothesis that Zn has a role in the function of biomembranes.

• Research Organization: Univ. of Guelph, Ontario
• OSTI ID: 7024519
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROGEN PEROXIDE
BIOSYNTHESIS
OXIDOREDUCTASES
ENZYME ACTIVITY
ZINC
BIOLOGICAL EFFECTS
NUTRITIONAL DEFICIENCY
CYTOCHROMES
DIET
LIVER
LUNGS
MICROSOMES
NADP
OXYGEN
RATS
ANIMALS
BODY
CELL CONSTITUENTS
COENZYMES
DIGESTIVE SYSTEM
ELEMENTS
ENZYMES
GLANDS
HYDROGEN COMPOUNDS
MAMMALS
METALS
NONMETALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXYGEN COMPOUNDS
PEROXIDES
PIGMENTS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology