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Title: Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation)

Abstract

The effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) synthetase, on potentially lethal damage repair (PLDR) was investigated in normal human fibroblasts and four human tumor cell lines from tumors with varying degrees of radiocurability. The tumor lines selected were: Ewing's sarcoma, a bone tumor considered radiocurable and, human lung adenocarcinoma, osteosarcoma, and melanoma, three tumors considered nonradiocurable. PLDR was measured by comparing cell survival when cells were irradiated in a density-inhibited state and replated at appropriate cell numbers at specified times following irradiation to cell survival when cells were replated immediately following irradiation. 3AB was added to cultures 2 hr prior to irradiation and removed at the time of replating. Different test radiation doses were used for the various cell lines to obtain equivalent levels of cell survival. In the absence of inhibitor, PLDR was similar in all cell lines tested. In the presence of 8 mM 3AB, differential inhibition of PLDR was observed. PLDR was almost completely inhibited in Ewing's sarcoma cells and partially inhibited in normal fibroblast cells and osteosarcoma cells. No inhibition of PLDR was observed in the lung adenocarcinoma or melanoma cells. Except for the osteosarcoma cells, inhibition of PLDR by 3AB correlated well withmore » radiocurability.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Georgetown Univ. Medical Center, Washington, DC
OSTI Identifier:
7023341
Resource Type:
Journal Article
Journal Name:
Int. J. Radiat. Oncol., Biol. Phys.; (United States)
Additional Journal Information:
Journal Volume: 8
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AMIDES; RADIOSENSITIVITY EFFECTS; OSTEOSARCOMAS; RADIOSENSITIVITY; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; FIBROBLASTS; IN VITRO; INHIBITION; NEOPLASMS; RADIOTHERAPY; SURVIVAL TIME; TUMOR CELLS; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; DISEASES; MEDICINE; NUCLEAR MEDICINE; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATION EFFECTS; RADIOLOGY; RECOVERY; REPAIR; SARCOMAS; SKELETAL DISEASES; SOMATIC CELLS; THERAPY; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Thraves, P J, Mossman, K L, Frazier, D T, and Dritschilo, A. Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation). United States: N. p., 1986. Web. doi:10.1016/0360-3016(86)90212-9.
Thraves, P J, Mossman, K L, Frazier, D T, & Dritschilo, A. Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation). United States. https://doi.org/10.1016/0360-3016(86)90212-9
Thraves, P J, Mossman, K L, Frazier, D T, and Dritschilo, A. 1986. "Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation)". United States. https://doi.org/10.1016/0360-3016(86)90212-9.
@article{osti_7023341,
title = {Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation)},
author = {Thraves, P J and Mossman, K L and Frazier, D T and Dritschilo, A},
abstractNote = {The effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) synthetase, on potentially lethal damage repair (PLDR) was investigated in normal human fibroblasts and four human tumor cell lines from tumors with varying degrees of radiocurability. The tumor lines selected were: Ewing's sarcoma, a bone tumor considered radiocurable and, human lung adenocarcinoma, osteosarcoma, and melanoma, three tumors considered nonradiocurable. PLDR was measured by comparing cell survival when cells were irradiated in a density-inhibited state and replated at appropriate cell numbers at specified times following irradiation to cell survival when cells were replated immediately following irradiation. 3AB was added to cultures 2 hr prior to irradiation and removed at the time of replating. Different test radiation doses were used for the various cell lines to obtain equivalent levels of cell survival. In the absence of inhibitor, PLDR was similar in all cell lines tested. In the presence of 8 mM 3AB, differential inhibition of PLDR was observed. PLDR was almost completely inhibited in Ewing's sarcoma cells and partially inhibited in normal fibroblast cells and osteosarcoma cells. No inhibition of PLDR was observed in the lung adenocarcinoma or melanoma cells. Except for the osteosarcoma cells, inhibition of PLDR by 3AB correlated well with radiocurability.},
doi = {10.1016/0360-3016(86)90212-9},
url = {https://www.osti.gov/biblio/7023341}, journal = {Int. J. Radiat. Oncol., Biol. Phys.; (United States)},
number = ,
volume = 8,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 1986},
month = {Fri Aug 01 00:00:00 EDT 1986}
}