In vitro growth characteristics of asbestos-induced diffused malignant mesotheliomas

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7022107

Akley, N; Mackay, A; Craighead, J

After long latency periods, DMM develop in rat inoculated into the pleural or peritoneal cavity with either chrysotile or crocidolite asbestos. Histologically, the tumors resemble the human lesion being either fibrosarcomatous or epithelial (or mixtures of the two cell types). Tumor tissue from most, but not all, lesions grow in serum containing medium in vitro. These tumor cells consistently are tetroploid or aneuploid; occasionally marker chromosomes are found. After a series of passages chemically defined serum-free medium maintains the growth of cells from many tumors in vitro. Cells in culture usually grow in monolayers but nodular masses of proliferating tumor cells develop from the cell sheet and readily float free in the medium. These seemingly spherical balls of cells can be used to establish fresh cultures, allowing the initial monolayers to grow indefinitely. The fine structural features of the nodular tumor masses have now been studied in detail. They consist of vacuolated epithelial cells which are replete with vellumentous villi. Experimentally-induced DMM in animals have characteristics similar to their human counterparts; implantation of metastases may develop from foci similar to those observed to form in cultures.

• Research Organization: Univ. of Vermont, Burlington
• OSTI ID: 7022107
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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CARCINOGENESIS
TUMOR CELLS
GROWTH
CELL PROLIFERATION
EXPERIMENTAL NEOPLASMS
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