Radioprotection of mice with interleukin-1: Relationship to the number of spleen colony-forming units
Compared to saline-injected mice 9 days after 6.5-Gy irradiation, there were twofold more Day 8 spleen colony-forming units (CFU-S) per femur and per spleen from B6D2F1 mice administered a radioprotective dose of human recombinant interleukin-1-alpha (rIL-1) 20 h prior to their irradiation. Studies of this report compared the numbers of CFU-S in nonirradiated mice 20 h after saline or rIL-1 injection. Prior to irradiation, the number of Day 8 CFU-S was not significantly different in the bone marrow or spleens from saline-injected mice and rIL-1-injected mice. Also, in the bone marrow, the number of Day 12 CFU-S was similar for both groups of mice. Similar seeding efficiencies for CFU-S and percentage of CFU-S in S phase of the cell cycle provided further evidence that rIL-1 injection did not increase the number of CFU-S prior to irradiation. In a marrow-repopulation assay, cellularity as well as the number of erythroid colony-forming units, erythroid burst-forming units, and granulocyte-macrophage colony-forming cells per femur of lethally irradiated mice were not increased in recipient mice of donor cells from rIL-1 injected mice. These results demonstrated that a twofold increase in the number of CFU-S at the time of irradiation was not necessary for the earlier recovery of CFU-S observed in mice irradiated with sublethal doses of radiation 20 h after rIL-1 injection.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 7018450
- Report Number(s):
- AD-A-214333/7/XAB; AFRRI-SR-89-38
- Resource Relation:
- Other Information: Pub. in Radiation Research, Vol. 119, 101-112(1989)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Recovery of hematopoietic colony-forming cells in irradiated mice pretreated with interleukin 1 (IL-1)
Recovery of hematopoietic colony-forming cells in irradiated mice pretreated with interleukin 1 (IL-1)
Related Subjects
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
HEMATOPOIETIC SYSTEM
RADIOPROTECTIVE SUBSTANCES
SPLEEN COLONY FORMATION
BLOOD CELLS
CELL CYCLE
CELL DIVISION
EFFICIENCY
IRRADIATION
LEGS
LEUKOCYTES
MACROPHAGES
MICE
PREVENTIVE MEDICINE
RADIATION PROTECTION
SPLEEN
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY AREAS
BODY FLUIDS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DRUGS
LIMBS
MAMMALS
MATERIALS
MEDICINE
ORGANS
PHAGOCYTES
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals