Temporal induction of cytochrome P450 1A in the mirror carp (Cyprinus carpio) after administration of several polycyclic aromatic hydrocarbons

Journal Article · Sun May 01 00:00:00 EDT 1994 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1897/1552-8618(1994)13[797:TIOCPA]2.0.CO;2· OSTI ID:7016340

Hanegraaf, F H.M.; Seinen, W; Berg, M Van Den ^[1]; Eggens, M L ^[2]; Celander, M ^[3]

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1. Univ. of Utrecht (Netherlands). Research Inst. of Toxicology
2. Ministry of Transport and Public Works, Haren (Netherlands). Dept. of Ecotoxicology
3. Univ. of Goeteborg (Sweden). Dept. of Zoophysiology

High inductions of 7-ethoxyresorufin O-deethylation (EROD) activities, cytochrome P450 1A protein levels (P4501A), and total cytochrome P450 content (P450) were observed 1, 2, 5, and 14 d after a single i.p. injection of 99.5 [mu]mol/kg benzo[a]pyrene (BaP) and chrysene (Chrys). The highest inductions in EROD activities and P450 1A protein levels were seen 2 d after treatment, approximately 35- and 11-fold relative to the controls. Less pronounced increase of EROD activities and P4501A protein levels, approximately 3- to 10-fold and 6-fold, respectively, were observed after treatments with equimolar concentrations of fluoranthenes (Fluor) and pyrene (Pyr). Benzo[ghi]perylene (BghiP) and the carrier dimethyl sulfoxide (DMSO) did not affect these parameters. The highest concentrations of PAHs in the liver, equivalent to 2 to 25% of the dose, were found 1 or 2 d after treatment. The highest percentage of the dose in the liver was observed for Chrys, followed by BghiP, BaP, Fluor, and Pyr. Poor correlations were found between hepatic concentrations of PAHs and P450 1A increases, which might be attributed to the rapid metabolism of these compounds.

• OSTI ID: 7016340
• Journal Information: Environmental Toxicology and Chemistry; (United States), Vol. 13:5; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CONDENSED AROMATICS
METABOLISM
FISHES
SENSITIVITY
OXYGENASES
ENZYME INDUCTION
BENZOPYRENE
CHRYSENE
PYRENE
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ENZYMES
GENE REGULATION
HYDROCARBONS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology