Fibre-optic-based fluoroimmunosensors

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Philosophical Transactions of the Royal Society of London. Series A, Physical Sciences and Engineering; (United Kingdom)

OSTI ID:7014205

Sepaniak, M J ^[1]; Vodinh, T ^[2]

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1. Univ. of Tennessee, Knoxville (United States)
2. Oak Ridge National Lab., TN (United States)

Remote sensing of chemicals can be performed using fibre optic chemical sensors that use immunochemical reagent phases. Exploiting the specificity of antibody-antigen interactions and the sensitivity of laser-excited fluorimetry, highly selective measurements of ultra-trace levels of chemicals can be performed remotely and in situ via fluoroimmunoassay techniques. In this work, heterogeneous assay protocols using immunobeads are implemented. A passive sensor that samples analyte by diffusion through a permeable membrane and is capable of a single analysis is described and used for the measurement of a naturally fluorescent compound. Subsequently, a regenerable sensor that can perform assay procedures in a repetitive fashion is described and characterized. The versatility of this sensor for performing remote measurements using a variety of established fluorimmunoassay methodologies is discussed.

• DOE Contract Number: FG05-86ER13613; AC05-84OR21400
• OSTI ID: 7014205
• Journal Information: Philosophical Transactions of the Royal Society of London. Series A, Physical Sciences and Engineering; (United Kingdom), Journal Name: Philosophical Transactions of the Royal Society of London. Series A, Physical Sciences and Engineering; (United Kingdom); ISSN 0962-8428
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOLOGICAL MATERIALS
CHEMICAL ANALYSIS
ANTIGEN-ANTIBODY REACTIONS
BENZOPYRENE
FLUORESCENCE SPECTROSCOPY
LASER RADIATION
MEMBRANES
METABOLITES
OPTICAL FIBERS
PROTEINS
REMOTE SENSING
TRACE AMOUNTS
AROMATICS
CONDENSED AROMATICS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
FIBERS
HYDROCARBONS
MATERIALS
ORGANIC COMPOUNDS
RADIATIONS
SPECTROSCOPY
550200* - Biochemistry
400102 - Chemical & Spectral Procedures