Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage
Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H-glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated.
- Research Organization:
- Massachusetts General Hospital, Boston, MA (United States)
- OSTI ID:
- 7013977
- Journal Information:
- Arthritis Rheum.; (United States), Vol. 27:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARTILAGE
CHEMICAL COMPOSITION
SULFATES
HYALURONIC ACID
BIOSYNTHESIS
RHEUMATIC DISEASES
ETIOLOGY
BIOCHEMICAL REACTION KINETICS
CENTRIFUGATION
LABELLED COMPOUNDS
MAN
MOLECULAR BIOLOGY
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CONNECTIVE TISSUE
DISEASES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PRIMATES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SKELETAL DISEASES
SULFUR COMPOUNDS
SYNTHESIS
TISSUES
VERTEBRATES
550901* - Pathology- Tracer Techniques