Ames assay chromatograms and the identification of mutagens in diesel particle extract
Dichloromethane extracts of passenger car diesel exhaust particles were separated into 65 fractions by normal-phase high-performance liquid chromatography (HPLC), and Ames assays (TA98 without activation) were carried out on each fraction. The only prominently mutagenic fractions were ones with elution times approximately coincident with those for 1,3-, 1,6-, and 1,8-dinitropyrenes, 1-nitropyrene, and 3- and 8-nitrofluoranthene. These compounds were quantified in one extract sample by gas chromatography-mass spectrometry and gas chromatography coupled with a nitrogen-phosphorus detector. Among five extract samples studied, 30-40% of the total mutagenicity recovered from the columns could be attributed to these six nitroarenes, 15-20% was recovered in the as yet uncharacterized polar fraction, and the remainder was distributed roughly equally over 63 fractions. About 70% of the mutagenicity applied to the HPLC columns was recovered from the columns. The Ames assay mutagenicities of the unfractionated extracts were shown to be additive in the mutagenicities of the components. 23 references.
- Research Organization:
- Ford Motor Co., Dearborn, MI
- OSTI ID:
- 7012674
- Journal Information:
- Environ. Sci. Technol.; (United States), Vol. 18:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIESEL FUELS
EXHAUST GASES
MUTAGEN SCREENING
NITRO COMPOUNDS
MUTAGENESIS
PYRENE
AUTOMOBILES
FRACTIONATION
GAS CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
PARTICULATES
SALMONELLA
AROMATICS
BACTERIA
CHROMATOGRAPHY
CONDENSED AROMATICS
FLUIDS
GASEOUS WASTES
GASES
HYDROCARBONS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLES
PETROLEUM PRODUCTS
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
VEHICLES
WASTES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)