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Title: Divergence of human [alpha]-chain constant region gene sequences: A novel recombinant [alpha]2 gene

Abstract

IgA is the major Ig synthesized in humans and provides the first line of defense at the mucosal surfaces. The constant region of IgA heavy chain is encoded by the [alpha] gene on chromosome 14. Previous studies have indicated the presence of two [alpha] genes, [alpha]1 and [alpha]2 existing in two allotypic forms, [alpha]2 m(1) and [alpha]2 m(2). Here the authors report the cloning and complete nucleotide sequence determination of a novel human [alpha] gene. Nucleotide sequence comparison with the published [alpha] sequences suggests that the gene arose as a consequence of recombination or gene conversion between the two [alpha]2 alleles. The authors have expressed the gene as a chimeric protein in myeloma cells indicating that it encodes a functional protein. The novel IgA resembles IgA2 m(2) in that disulfide bonds link H and L chains. This novel recombinant gene provides insights into the mechanisms of generation of different constant regions and suggests that within human populations, multiple alleles of [alpha] may be present providing IgAs of different structures.

Authors:
;  [1];  [2]
  1. Univ. of California, Los Angeles, CA (United States)
  2. UCLA School of Medicine, Los Angeles, CA (United States)
Publication Date:
OSTI Identifier:
7009416
Resource Type:
Journal Article
Journal Name:
Journal of Immunology; (United States)
Additional Journal Information:
Journal Volume: 152:11; Journal ID: ISSN 0022-1767
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; DNA SEQUENCING; DNA-CLONING; IMMUNOGLOBULINS; HUMAN CHROMOSOME 14; CHROMOSOMES; CLONING; DNA HYBRIDIZATION; GLOBULINS; HUMAN CHROMOSOMES; HYBRIDIZATION; ORGANIC COMPOUNDS; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Chintalacharuvu, K R, Morrison, S L, and Raines, M. Divergence of human [alpha]-chain constant region gene sequences: A novel recombinant [alpha]2 gene. United States: N. p., 1994. Web.
Chintalacharuvu, K R, Morrison, S L, & Raines, M. Divergence of human [alpha]-chain constant region gene sequences: A novel recombinant [alpha]2 gene. United States.
Chintalacharuvu, K R, Morrison, S L, and Raines, M. 1994. "Divergence of human [alpha]-chain constant region gene sequences: A novel recombinant [alpha]2 gene". United States.
@article{osti_7009416,
title = {Divergence of human [alpha]-chain constant region gene sequences: A novel recombinant [alpha]2 gene},
author = {Chintalacharuvu, K R and Morrison, S L and Raines, M},
abstractNote = {IgA is the major Ig synthesized in humans and provides the first line of defense at the mucosal surfaces. The constant region of IgA heavy chain is encoded by the [alpha] gene on chromosome 14. Previous studies have indicated the presence of two [alpha] genes, [alpha]1 and [alpha]2 existing in two allotypic forms, [alpha]2 m(1) and [alpha]2 m(2). Here the authors report the cloning and complete nucleotide sequence determination of a novel human [alpha] gene. Nucleotide sequence comparison with the published [alpha] sequences suggests that the gene arose as a consequence of recombination or gene conversion between the two [alpha]2 alleles. The authors have expressed the gene as a chimeric protein in myeloma cells indicating that it encodes a functional protein. The novel IgA resembles IgA2 m(2) in that disulfide bonds link H and L chains. This novel recombinant gene provides insights into the mechanisms of generation of different constant regions and suggests that within human populations, multiple alleles of [alpha] may be present providing IgAs of different structures.},
doi = {},
url = {https://www.osti.gov/biblio/7009416}, journal = {Journal of Immunology; (United States)},
issn = {0022-1767},
number = ,
volume = 152:11,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 1994},
month = {Wed Jun 01 00:00:00 EDT 1994}
}