Role of oxygen free radicals in the induction of sister chromatid exchanges by cigarette smoke

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130107· OSTI ID:7006569

Lee, C K; Brown, B G; Rice, Jr, W Y; Doolittle, D J ^[1]

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1. R.J. Reynolds Tobacco Co., Winston-Salem, NC (USA)

Cigarette smoke has been reported to contain free radicals and free radical generators in both the gas and particulate phases. Studies in our laboratory have shown that both cigarette smoke condensate (CSC) and smoke bubbled through phosphate buffered saline solution (smoke-PBS) increased sister chromatid exchanges (SCE) in Chinese hamster ovary cells in a dose-dependent manner. Since oxygen free radicals have been shown to cause SCEs and other chromosomal damage, we investigated the role of these radicals in the induction of SCEs by CSC and smoke-PBS. Addition of the antioxidant enzymes catalase and superoxide dismutase or the oxygen-radical scavenger ascorbic acid failed to reduce the SCE frequency in the presence of either CSC or smoke-PBS. Additional studies indicated that the quantity of hydrogen peroxide produced in CSC or smoke-PBS is too small to account for the observed SCE induction. It appears, therefore, that SCE induction by CSC or smoke-PBS does not involve the participation of oxygen free radicals.

• OSTI ID: 7006569
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Vol. 13:1; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHO CELLS
SISTER CHROMATID EXCHANGES
CHROMOSOMES
GENETIC RADIATION EFFECTS
HYDROXYL RADICALS
BIOCHEMISTRY
TOBACCO SMOKES
GENETIC EFFECTS
ANTIOXIDANTS
CARBON 14 COMPOUNDS
SUPEROXIDE DISMUTASE
X RADIATION
AEROSOLS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMISTRY
CHROMOSOMAL ABERRATIONS
COLLOIDS
DISPERSIONS
ELECTROMAGNETIC RADIATION
ENZYMES
IONIZING RADIATIONS
LABELLED COMPOUNDS
MUTATIONS
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
RADICALS
RESIDUES
SMOKES
SOLS
560120* - Radiation Effects on Biochemicals
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