Gradient elution for micellar electrokinetic capillary chromatography

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00158a003· OSTI ID:7005557

Balchunas, A T; Sepaniak, M J

A method for performing gradient elution in micellar electrokinetic capillary chromatography is developed. The influence of temperature and mobile phase organic solvent concentration on capacity factors is presented and attributed to changes in phase ratio and solute distribution coefficient. Solvent programming is more effective than temperature programming at adjusting retention due to its greater influence on distribution coefficient. As anticipated, phase ratio changes could not be exploited due to concomitant losses in column efficiency. A stepwise solvent gradient involving increasing concentrations of 2-propanol and Triton-X-100 is used for the separation of a test mixture of derivatized amines.

• Research Organization: Univ. of Tennessee, Knoxville (USA)
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 7005557
• Journal Information: Anal. Chem.; (United States), Vol. 60:7
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
AMINES
LIQUID COLUMN CHROMATOGRAPHY
CAPILLARIES
CHEMICAL REACTION KINETICS
DISTRIBUTION FUNCTIONS
EFFICIENCY
ELECTRODYNAMICS
ELUTRIATION
EXPERIMENTAL DATA
MICELLAR SYSTEMS
ORGANIC SOLVENTS
PHASE STUDIES
QUANTITY RATIO
SOLUTES
TEMPERATURE DEPENDENCE
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
DATA
FUNCTIONS
INFORMATION
KINETICS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SEPARATION PROCESSES
SOLVENTS
400105* - Separation Procedures