Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
This patent describes a composition. It comprises the structure: S-L(NA{sub 1}-S-NA{sub 2}){sub n}M. NA{sub 1} and NA{sub 2} are nucleic acid sequences; -S- is a scissile linkage which is capable of being cleaved or disrupted without cleaving or disrupting the nuclei acid sequences of NA{sub 1} or NA{sub 2} or of a target nuclei acid sequence capable of hybridizing to the NA{sub 1} and NA{sub 2} sequences, or to the NA{sub 1} and NA{sub 2} sequences and the scissile linkage of the composition, wherein if the scissile linkage is a nuclei acid sequence it is RNA when both NA{sub 1} and NA{sub 2} are DNA sequences, or the scissile linkage is DNA when both NA{sub 1} and NA{sub 2} are RNA sequences; and n is an integer from 1 to 4. The solid lines represent chemical bonds; X is a solid support; L is a chemical entity which links NA{sub 1} to the solid support; and M is a marker.
- Assignee:
- Meiogenics, Inc., Columbia, MD
- Patent Number(s):
- US 4876187
- Application Number:
- PPN: US 6805279A
- OSTI ID:
- 7003398
- Resource Relation:
- Patent File Date: 5 Dec 1985
- Country of Publication:
- United States
- Language:
- English
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