Insulin effect on (/sup 14/C)-valine incorporation and its relation to hexokinase activity in developing brain
Using minced brain cortex from fetal and postnatal rats, we studied the incorporation of (/sup 14/C)-valine into protein in the presence of insulin. We also assayed the particle bound and soluble hexokinase in these tissues. Insulin significantly stimulated the incorporation of (/sup 14/C)-valine into brain proteins from fetal stage upto 2 days of life. After this period the insulin effect was minimal, with no effect by day 5. The particle bound (40,000g pellet) brain hexokinase, on the other hand, remained low till about 2 days of life and then increased to almost adult level by 5 days. Our results show that there is an inverse relation between this anabolic effect of insulin and the particle bound hexokinase activity in the cortex of developing rat brain.
- Research Organization:
- Univ. of Southern California, Los Angeles (USA)
- OSTI ID:
- 7002585
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 154:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Measurement of local rates of brain protein synthesis by quantitative autoradiography: validation with L-(/sup 3/H)valine
Transfer coefficients for L-valine and the rate of incorporation of L-(1-/sup 14/C) valine into proteins in normal adult rat brain
Related Subjects
HEXOKINASE
ENZYME ACTIVITY
INSULIN
BIOLOGICAL EFFECTS
VALINE
METABOLISM
AGE DEPENDENCE
CARBON 14 COMPOUNDS
CEREBRAL CORTEX
RATS
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CEREBRUM
ENZYMES
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RODENTS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques