Fluorography of tritium-labeled proteins in silver-stained polyacrylamide gels
Journal Article
·
· Anal. Biochem.; (United States)
Silver-staining of polyacrylamide gel electrophoresis (PAGE)-separated proteins allows sensitive detection of proteins but severely reduces the ability to detect weak beta-emitters present in the protein band. A simple procedure is described in which silver can be removed from a silver-stained PAGE gel (deargentation) using photographic fixer, and the silver-free gel can be enhanced and used for fluorography. A quantitative study of sensitivity is reported for /sup 3/H-labeled bovine serum albumin with a one-dimensional sodium dodecyl sulfate-PAGE slab gel.
- Research Organization:
- State Univ. of New York, Stony Brook (USA)
- OSTI ID:
- 7002445
- Journal Information:
- Anal. Biochem.; (United States), Vol. 170:2
- Country of Publication:
- United States
- Language:
- English
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