Protein synthesis rates in rat brain regions and subcellular fractions during aging

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00972483· OSTI ID:7002411

Avola, R; Condorelli, D F; Ragusa, N; Renis, M; Alberghina, M; Giuffrida Stella, A M; Lajtha, A

In vivo protein synthesis rates in various brain regions (cerebral cortex, cerebellum, hippocampus, hypothalamus, and striatum) of 4-, 12-, and 24-month-old rats were examined after injection of a flooding dose of labeled valine. The incorporation of labeled valine into proteins of mitochondrial, microsomal, and cytosolic fractions from cerebral cortex and cerebellum was also measured. At all ages examined, the incorporation rate was 0.5% per hour in cerebral cortex, cerebellum, hippocampus, and hypothalamus and 0.4% per hour in striatum. Of the subcellular fractions examined, the microsomal proteins were synthesized at the highest rate, followed by cytosolic and mitochondrial proteins. The results obtained indicate that the average synthesis rate of proteins in the various brain regions and subcellular fractions examined is fairly constant and is not significantly altered in the 4 to 24-month period of life of rats.

• Research Organization: Univ. of Catania (Italy)
• OSTI ID: 7002411
• Journal Information: Neurochem. Res.; (United States), Vol. 13:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
AGE DEPENDENCE
BRAIN
IN VIVO
LABELLED COMPOUNDS
MICROSOMES
MITOCHONDRIA
RATS
SUBCELLULAR DISTRIBUTION
TRACER TECHNIQUES
VALINE
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques