skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microbial activity of trench leachates from shallow-land, low-level radioactive waste disposal sites

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:7001683

Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobacter sp., and Clostridium sp. were identified. Mixed bacterial cultures isolated from the trench leachates were able to grow anaerobically in trench leachates, which indicates that the radionuclides and organic chemicals present were not toxic to these bacteria. Changes in concentrations of several of the organic constituents of the waste leachate samples were observed due to anaerobic microbial activity. Growth of a mixed culture of trench-water bacteria in media containing a mixture of radionuclides, /sup 60/Co, /sup 85/Sr, and /sup 134/ /sup 137/Cs, was not affected at total activity concentrations of 2.6 x 10/sup 2/ and 2.7 x 10/sup 3/ pCi/ml.

Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
7001683
Journal Information:
Appl. Environ. Microbiol.; (United States), Vol. 40:1
Country of Publication:
United States
Language:
English