Pluripotent stem cells with normal or reduced self renewal survive lethal irradiation

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Exp. Hematol.; (United States)

OSTI ID:6999301

Brecher, G; Neben, S; Yee, M; Bullis, J; Cronkite, E P

Transfusion with 10,000 or 20,000 marrow cells resulted in 30+ days survival of 15%-50% of mice exposed to an Ld90 or LD100 or radiation. The use of congenic mice with alloenzyme markers permitted the identification of host and donor cells in the peripheral blood of transfused animals. Donor cells were present initially in all hosts. Between 55% and 92% of the animals became 100% host type by 12-24 weeks after transfusion in three separate experiments. To explore whether the temporary repopulation by donor cells was due to short-lived stem cells, the marrows of several primary hosts were transfused into secondary, lethally irradiated hosts. Some of the retransplanted primary donor and host cells persisted only temporarily. It is suggested that some of the donor stem cells in both the primary and secondary hosts had an intrinsically shortened life span.

• Research Organization: Lawrence Berkeley Lab., Berkeley (USA)
• OSTI ID: 6999301
• Journal Information: Exp. Hematol.; (United States), Vol. 16:7
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
TRANSPLANTS
STEM CELLS
SURVIVAL TIME
CELL DIVISION
LETHAL IRRADIATION
MICE
RADIATION CHIMERAS
TRANSFUSIONS
ANIMAL CELLS
ANIMALS
CHIMERAS
CONNECTIVE TISSUE CELLS
IRRADIATION
MAMMALS
MOSAICISM
RODENTS
SOMATIC CELLS
THERAPY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals