Transcriptional and translational analyzes of recA mutant alleles in Pseudomonas aeruginosa

Journal Article · Fri Apr 01 00:00:00 EST 1988 · J. Bacteriol.; (United States)

OSTI ID:6998615

Horn, J M; Ohman, D E

Recombinant plasmids containing the recA gene from Pseudomonas aeruginosa were used in complementation, transcriptional, and translational studies to examine the nature of rec-102 and rec-2, mutations which confer a recA-like mutant phenotype on P. aeruginosa PAO strains. For comparison, recA7::Tn501 mutants of strain PAO were constructed by gene replacement. The rec-2 and rec-102 alleles were shown to be recA alleles; plasmids containing the recA gene complemented the three rec mutants strains for defects associated with recA mutation. A minicell analysis showed that a plasmid expressing both of the recA gene transcripts or one that expressed only the smaller transcript both produced the same 42-kilodalton recA protein. A chloramphenicol acetyl-transferase gene fusion in the 3' end of the recA transcript showed that the recA gene of P. aeruginosa was induced following treatment with a DNA-damaging agent (methyl methanesulfonate). The rec-2 allele of recA was normal with respect to induction of mRNA, but these transcripts were defective in either translation or synthesis of a stable protein. The rec-102 mutant also produced a detectable transcript and no recA protein following induction. The recA defect in this mutant is apparently in the interaction between recA and a lexA-like repressor.

• Research Organization: Univ. of California, Berkeley (USA)
• OSTI ID: 6998615
• Journal Information: J. Bacteriol.; (United States), Vol. 170:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPAIR
MOLECULAR BIOLOGY
METHYL METHANESULFONATE
MUTAGENESIS
PSEUDOMONAS
GENE REGULATION
GENE REPRESSORS
MUTANTS
PLASMIDS
RECOMBINANT DNA
TRANSCRIPTION
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA
ESTERS
MICROORGANISMS
MUTAGENS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RECOVERY
REPAIR
SULFONIC ACID ESTERS
550200* - Biochemistry
560300 - Chemicals Metabolism & Toxicology