Reaction of ferric heme proteins with nitrite and sulfite

Journal Article · Tue Apr 19 00:00:00 EDT 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00408a020· OSTI ID:6997412

Young, L J; Siegel, L M

Optical and EPR spectroscopy of ferric heme proteins of the porphyrin, oxyporphyrin, and isobacteriochlorin classes has indicated that nitrite reacts with these proteins at the heme iron. Sulfite has been conclusively proven to react only with proteins containing the isobacteriochlorin macrocycle. Quantitative EPR spectroscopy of these nitrite and sulfite adducts showed that most contained a substantial quantity of undetectable heme. It is suggested that protein-induced autoreduction of nitrite (but not sulfite) and a strained and/or uniaxial g-tensor are the principal ways by which the silent state is produced.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA)
• OSTI ID: 6997412
• Journal Information: Biochemistry; (United States), Vol. 27:8
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NITRITES
BIOLOGICAL EFFECTS
PORPHYRINS
VISIBLE SPECTRA
SULFITES
ELECTRON SPECTRA
ELECTRON SPIN RESONANCE
HEME
OXIDOREDUCTASES
PURIFICATION
CARBOXYLIC ACIDS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAGNETIC RESONANCE
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PIGMENTS
RESONANCE
SPECTRA
SULFUR COMPOUNDS
560300* - Chemicals Metabolism & Toxicology