Bovine placental lactogen: isolation purification and measurement in biological fluids
Studies were conducted to isolate and purify bovine placental lactogen (bPL) and to develop a radioimmunoassay to this protein. Bovine placental lactogen was isolated from culture medium after a 24 hr culture of fetal cotyledonary tissue. Cotyledonary explants were stimulated to secrete bPL by either addition of bovine growth hormone (NIH-B8) to the medium or co-culture of cotyledon and caruncular tissue. Production of bPL was greatly affected by explant size and 70% of that produced in a 48 hr culture was released in the first 12 hr. Purification of bPL was accomplished using a column chromatographic scheme that involved gel filtration, ion exchange and chromatofocusing chromatography. A radioimmunoassay to bPL was developed using an antibody raised at the USDA Beltsville (F56). Dose response curves of amniotic or allantoic fluid or fetal and maternal serum were parallel to the standard curve and bPL was quantitatively recovered at from 82-125%. Using the radioimmunoassay, samples of amniotic and allantoic fluids and fetal and maternal serum were measured for bPL. Concentrations of bPL ranged from undetectable to 50 ng/ml, with fetal blood having the highest concentrations and amniotic fluid the lowest.
- Research Organization:
- Florida Univ., Gainesville (USA)
- OSTI ID:
- 6997017
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LACTOGENS
PURIFICATION
RADIOIMMUNOASSAY
AMNIOTIC FLUID
BLOOD SERUM
CATTLE
FETUSES
FILTRATION
ION EXCHANGE CHROMATOGRAPHY
PLACENTA
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
DOMESTIC ANIMALS
FETAL MEMBRANES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MEMBRANES
RADIOASSAY
RADIOIMMUNOLOGY
RUMINANTS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics