Extraction of pectins and hemicelluloses from cell walls of suspension culture cells of cotton by endopolygalacturonase treatment

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6995410

An, Jinhua; Maness, N O; Mort, A J ^[1]

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1. Oklahoma State Univ., Stillwater (USA)

Walls of cotton suspension cultures were treated with a highly purified endopolygalacturonase coded for by a gene from Erwinia carotovora expressed in E. coli. About 80 percent of the walls galacturonic acid was solubilized and could be fractionated by ion exchange chromatography into three classes: (1) tri- and tetra-galacturonides from digestion of homogalacturonans, (2) high molecular weight fragments rich in galacturonic acid, rhamnose and other neutral sugars indicative of rhamnogalacturonan I, and (3) a well defined low molecular weight polymer resembling rhamnogalacturonan II. Treatment of the insoluble wall residue with alkali allowed extraction of the remainder of the wall galacturonic acid as high molecular weight polymers resembling rhamnogalacturonan I but in association with what is probably xyloglucan. The purified polymers will be compared to those obtained by others from different species, especially those of sycamore.

• OSTI ID: 6995410
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL WALL
BIOCHEMISTRY
BACTERIA
COTTON PLANTS
ESCHERICHIA COLI
GALACTURONIC ACID
GENES
GLYCOSYL HYDROLASES
HEMICELLULOSE
PECTINS
ALDEHYDES
BLOOD SUBSTITUTES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DRUGS
ENZYMES
HEMATOLOGIC AGENTS
HYDROLASES
HYDROXY ACIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
550200* - Biochemistry