Immunocytochemical localization of the vacuolar H sup + -ATPase in maize root tip cells. [Zea mays]

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.2.391· OSTI ID:6995348

Hurley, D; Taiz, L ^[1]

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1. Univ. of California, Santa Cruz (USA)

The vacuolar H{sup +}-ATPase of maize (Zea mays L.) root tip cells has been localized at the EM level using rabbit polyclonal antibodies to the 69 kilodalton subunit and protein A-colloidal gold. Intracellular gold particles were detected mainly on the tonoplast and Golgi membranes. Only about 27% of the vacuoles were labeled above background. The absence of gold particles on the majority of vacuoles suggests either that the tonoplast H{sup +}-ATPase is degraded during tissue preparation or that the small vacuoles of root tip cells are specialized with respect to H{sup +}-ATPase activity. The pattern of gold particles on the labeled vacuoles ranged from uniform to patchy. Virtually all of the Golgi bodies were labeled by the antibody, but the particle densities were too low to determine whether the H{sup +}-ATPase was associated with specific regions, such as the trans-face. Cell wall-labeling was also observed which could be partially prevented by the inclusion of gelatin as a blocking agent. The immunocytochemical results confirm previous biochemical studies with isolated membrane fractions.

• DOE Contract Number: FG03-84ER13245
• OSTI ID: 6995348
• Journal Information: Plant Physiology; (USA), Vol. 89:2; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PLANT CELLS
ENZYME IMMUNOASSAY
ATP-ASE
CELL WALL
CYTOLOGY
ELECTRON MICROSCOPY
ENZYME ACTIVITY
MAIZE
ROOTS
ACID ANHYDRASES
BIOASSAY
BIOLOGY
CELL CONSTITUENTS
CEREALS
ENZYMES
GRASS
HYDROLASES
IMMUNOASSAY
LILIOPSIDA
MAGNOLIOPHYTA
MICROSCOPY
PHOSPHOHYDROLASES
PLANTS
550200* - Biochemistry