Gastrin-releasing peptide stimulates glycoconjugate release from feline trachea

Journal Article · Thu Feb 01 00:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:6994985

Lundgren, J D; Baraniuk, J N; Ostrowski, N L; Kaliner, M A; Shelhamer, J H ^[1]

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1. National Institute of Allergy and Infectious Diseases, Bethesda, MD (USA)

The effect of gastrin-releasing peptide (GRP) on respiratory glycoconjugate (RGC) secretion was investigated in a feline tracheal organ culture model. RGC secretion was stimulated by GRP in a dose-dependent fashion at concentrations from 10(-8) to 10(-5) M (range 15-38% increase above control) with a peak effect within 0.5-1 h of incubation. GRP-(14-27), the receptor binding portion of GRP, and the related molecule, bombesin, also stimulated RGC secretion by approximately 20% above control. Acetyl-GRP-(20-27) stimulated RGC release by 10%, whereas GRP-(1-16) was inactive. Autoradiographic studies with 125I-GRP revealed that specific binding was restricted to the submucosal glands and the surface epithelium. A specific radioimmunoassay showed the content of GRP in feline trachea after extraction with ethanol-acetic acid to be 156 +/- 91 fmol/g wet wt. Indirect immunohistochemistry indicated that ganglion cells located just outside the cartilage contained GRP-immunoreactive materials. GRP is a novel mucus secretagogue that may participate in regulating airway mucosal gland secretion.

• OSTI ID: 6994985
• Journal Information: American Journal of Physiology; (USA), Vol. 258; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
SECRETION
PEPTIDES
BIOCHEMICAL REACTION KINETICS
AUTORADIOGRAPHY
CATS
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
IODINE 125
MUSCLES
RADIOIMMUNOASSAY
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACHEA
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BODY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques