Evolutionary, environmental and tissue controls on the occurrence of multiple isoforms of acyl carrier protein

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6993075

Battey, J F; Ohlrogge, J B ^[1]

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1. Michigan State Univ., East Lansing (USA)

Previous research has revealed that several higher plant species have multiple isoforms of acyl carrier protein (ACP). We have examined the development of this trait in evolutionarily diverse species. Isoforms were resolved by Western blotting and native PAGE of {sup 3}H-palmitate labelled ACP's. Multiple isoforms of ACP were observed in primitive vascular plants including gymnosperms, ferns and Psilotum and the nonvascular liverworts and mosses. Therefore, the development of ACP isoforms occurred early in evolution. However, unicellular algae and bacteria such as Chlamydomonas, Dunaliella, Synechocystis and Agmnellum have only a single electrophoretic form of ACP. Thus, multiple forms of ACP do not occur in all photosynthetic organisms but may be associated with multicellular plants. We have also examined light and tissue control over the expression of ACP isoforms. The expression of multiple forms of ACP in leaf of Spinacia and Avena is altered very little by light. Rather, the different patterns of ACP isoforms are primarily dependant on tissue source.

• OSTI ID: 6993075
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PLANTS
PHYSIOLOGY
ALGAE
BACTERIA
FERNS
GRASS
MOSSES
PHOTOSYNTHESIS
PROTEIN STRUCTURE
SPINACH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BRYOPHYTA
CHEMICAL REACTIONS
FOOD
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
PHOTOCHEMICAL REACTIONS
SYNTHESIS
VEGETABLES
551001* - Physiological Systems- Tracer Techniques