UV-induced synthesis of hydrogen peroxide

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6992668

Murphy, T M; Huerta, A J ^[1]

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1. Univ. of California, Davis (USA)

Suspension-cultured rose cells irradiated with UV (254 mm, 558 J m{sup {minus}2}) showed a transient efflux of K{sup +}, and a production of H{sub 2}O{sub 2} measured by chemiluminescence of luminol in the presence of peroxidase. The peak concentration of H{sub 2}O{sub 2}, attained at about 60-90 min after irradiation, was 2-5 uM. The addition of superoxide dismutase to irradiated cells stimulated luminscence, suggesting that the H{sub 2}O{sub 2} came at least in part from superoxide that was present in the extracellular medium. Treatments that inhibited the UV-induced efflux of K{sup +} also inhibited the appearance of H{sub 2}O{sub 2}, though the converse was not always true, suggesting that K{sup +} efflux was necessary for H{sub 2}O{sub 2} synthesis, but not vice-versa. H{sub 2}O{sub 2} in the extracellular space is required for lignin synthesis in many plant tissues. Phenolic compounds, the other substrates for lignin, are induced by UV. We suggest that the UV-stimulated production of H{sub 2}O{sub 2} is part of a coordinated induction of lignin synthesis.

• OSTI ID: 6992668
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROGEN PEROXIDE
BIOSYNTHESIS
PLANT CELLS
BIOLOGICAL RADIATION EFFECTS
LIGNIN
LUMINESCENCE
POTASSIUM COMPOUNDS
SUPEROXIDE DISMUTASE
ULTRAVIOLET RADIATION
ALKALI METAL COMPOUNDS
BIOLOGICAL EFFECTS
CARBOHYDRATES
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
POLYSACCHARIDES
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SYNTHESIS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture