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Title: Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure

Abstract

The mechanism by which acute ethanol (ET) exposure causes reversible myocardial dysfunction is unknown. The purpose of this study was to examine the effects of ET exposure on contraction and cytosolic free Ca[sup 2+] ([Ca[sup 2+]][sub i]) in electrically-stimulated single rat ventricular myocytes loaded with the fluorescent Ca[sup 2+] indicator, fura-2. Whole cell fluorescence images were obtained at high speed with a frame transfer CCD camera and processed by a digital imaging system which allowed simultaneous measurement of cell shortening and [Ca[sup 2+]][sub i] changes. A concentration- and time-dependent decline in cell shortening was observed during a 20 min period of ET perfusion. At 3% ET, maximal shortening was reduced by 65%. Cell shortening was restored following washout of the ET. In parallel with the depression of contractility, the magnitude of the [Ca[sup 2+]][sub i] transient was also reduced. ET had no significant effect on basal [Ca[sup 2+]][sub i] at concentrations up to 3%. Kinetic parameters of the [Ca[sup 2+]][sub i] transient, including rates of rise and decay were also depressed, although the time to peak was unaffected by ET. These results indicate that ET interferes with Ca[sup 2+] fluxes in the ventricular myocyte in a manner that may contributemore » to the contractile impairment of cardiac function induced by acute ET exposure in vivo.« less

Authors:
; ;  [1]
  1. Thomas Jefferson Univ., Philadelphia, PA (United States)
Publication Date:
OSTI Identifier:
6992331
Report Number(s):
CONF-9202109-
Journal ID: ISSN 0892-6638; CODEN: FAJOEC
Resource Type:
Conference
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
Additional Journal Information:
Journal Volume: 6:1; Conference: American Society for Biochemistry and Molecular Biology and Biophysical Society joint meeting, Houston, TX (United States), 9-13 Feb 1992; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CALCIUM COMPOUNDS; MEMBRANE TRANSPORT; ETHANOL; BIOLOGICAL EFFECTS; HEART; SENSITIVITY; ACUTE EXPOSURE; CONTRACTION; DOSE-RESPONSE RELATIONSHIPS; TIME DEPENDENCE; ALCOHOLS; ALKALINE EARTH METAL COMPOUNDS; BODY; CARDIOVASCULAR SYSTEM; HYDROXY COMPOUNDS; ORGANIC COMPOUNDS; ORGANS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Rozanski, D J, Delaville, F J, and Thomas, A P. Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure. United States: N. p., 1992. Web.
Rozanski, D J, Delaville, F J, & Thomas, A P. Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure. United States.
Rozanski, D J, Delaville, F J, and Thomas, A P. 1992. "Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure". United States.
@article{osti_6992331,
title = {Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure},
author = {Rozanski, D J and Delaville, F J and Thomas, A P},
abstractNote = {The mechanism by which acute ethanol (ET) exposure causes reversible myocardial dysfunction is unknown. The purpose of this study was to examine the effects of ET exposure on contraction and cytosolic free Ca[sup 2+] ([Ca[sup 2+]][sub i]) in electrically-stimulated single rat ventricular myocytes loaded with the fluorescent Ca[sup 2+] indicator, fura-2. Whole cell fluorescence images were obtained at high speed with a frame transfer CCD camera and processed by a digital imaging system which allowed simultaneous measurement of cell shortening and [Ca[sup 2+]][sub i] changes. A concentration- and time-dependent decline in cell shortening was observed during a 20 min period of ET perfusion. At 3% ET, maximal shortening was reduced by 65%. Cell shortening was restored following washout of the ET. In parallel with the depression of contractility, the magnitude of the [Ca[sup 2+]][sub i] transient was also reduced. ET had no significant effect on basal [Ca[sup 2+]][sub i] at concentrations up to 3%. Kinetic parameters of the [Ca[sup 2+]][sub i] transient, including rates of rise and decay were also depressed, although the time to peak was unaffected by ET. These results indicate that ET interferes with Ca[sup 2+] fluxes in the ventricular myocyte in a manner that may contribute to the contractile impairment of cardiac function induced by acute ET exposure in vivo.},
doi = {},
url = {https://www.osti.gov/biblio/6992331}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
issn = {0892-6638},
number = ,
volume = 6:1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}

Conference:
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