Age related changes in steroid receptors on cultured lung fibroblasts
The number of high affinity glucocorticoid receptors (Ro) on human fetal lung fibroblasts decreases as the cells age in vitro, and it has been suggested that these cell systems may be useful models of age-related changes in vivo. They examined the relation between change in Ro with in vitro aging and donor age. Confluent monolayers of lung fibroblasts at various population doubling levels (PDL), were incubated with (/sup 3/H)-dexamethasone ((/sup 3/H)Dex) either alone or with excess (.01 mM) Dex. Specific binding was calculated as the difference between radioactivity in cells incubated with and without unlabeled Dex; Scatchard plots were used to analyze the data. Ro, measured as fmol (/sup 3/H)Dex/10/sup 6/ cells, for two lines of human fetal cells (HFL-1 and MRC-5) decreased with increasing age in vitro. However, human newborn (CRL-1485) and adult (CCL-201) cells and fetal rabbit cells (FAB-290), showed increases in Ro with continuous passage. For each cell line, the affinity constant (K/sub d/) did not change significantly with passage. They conclude that the direction of changes in steroid receptor levels on cells aging in vitro is influenced by donor age and species. Caution should be used in applying results obtained from model systems to aging organisms.
- Research Organization:
- City Univ. of New York, Jamaica
- OSTI ID:
- 6991941
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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GLUCOCORTICOIDS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
ADULTS
AFFINITY
AGE DEPENDENCE
BIOLOGICAL MODELS
BIOLOGICAL VARIABILITY
DEXAMETHASONE
FETUSES
FIBROBLASTS
INFANTS
LUNGS
MAN
RABBITS
TRITIUM COMPOUNDS
ADRENAL HORMONES
AGE GROUPS
ANIMAL CELLS
ANIMALS
BODY
CHILDREN
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PRIMATES
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques